Journal of Spectral Imaging,   Volume 9   Article ID a3   (2020)

Peer reviewed Paper

Part of Spectral Imaging in Synchrotron Light Facilities Special Issue

New insights in osteocyte imaging by synchrotron radiation

  • H. Portier
  • C. Jaffré
  • C.M. Kewish
  • C. Chappard
  • S. Pallu
Université de Paris, CNRS, Inserm, B3OA, F-75010 Paris, France and Université d’Orléans, COST, 45100 Orléans, France

 https://orcid.org/0000-0003-3328-3257
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Université de Paris, CNRS, Inserm, B3OA, F-75010 Paris, France and Faculté des Sciences du Sport et Laboratoire Adaptations Physiologiques à l’Exercice et Réadaptation à l’effort (APERE)-EA3300, Allée Pascal Grousset, 80025 Amiens Cedex 1, France

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Australian Nuclear Science and Technology Organisation (ANSTO), Australian Synchrotron, Clayton, VIC 3800, Australia and Department of Chemistry and Physics, La Trobe University, Bundoora, VIC 3086, Australia

 https://orcid.org/0000-0001-6242-7059
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Université de Paris, CNRS, Inserm, B3OA, F-75010 Paris, France

 https://orcid.org/0000-0001-9088-1582
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 Corresponding Author
Université de Paris, CNRS, Inserm, B3OA, F-75010 Paris, France and Université d’Orléans, COST, 45100 Orléans, France
[email protected]
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Bone health depends on the bone mineral density and mechanical strength, characterised quantitatively and inferred through qualitative parameters such as the trabecular and cortical micro-architecture, and other parameters describing the bone cells. Among these cells, the osteocyte has been recognised as the orchestrator of bone remodelling, playing a key role in directing osteoblastic and osteoclastic activities. Conventional optical and electron microscopies have greatly improved our understanding of the cell physiology mechanisms involved in different osteoarticular pathophysiological contexts, especially osteoporosis. More recently, imaging methods exploiting synchrotron radiation, such as X-ray tomography, ptychography, and deep ultraviolet and Fourier transform infrared spectroscopies, have revealed new biochemical, chemical and 3D morphological information about the osteocyte lacuna, the surrounding matrix and the lacuna–canalicular network at spatial length scales spanning microns to tens of nanometres. Here, we review recent results in osteocyte lacuna and lacuna–canalicular network characterisation by synchrotron radiation imaging in human and animal models, contributing to new insights in different physiologic and pathophysiological situations.

Keywords: osteocyte imaging, synchrotron radiation, spectroscopy, tomography

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