Journal of Spectral Imaging,   Volume 9   Article ID a1   (2020)

Peer reviewed Paper

Unstained blood smear contrast enhancement using spectral time multiplexing super resolution

  • Marie-Florence A. Yebouet
  • Ambroise K. Diby
  • Kenneth A. Kaduki
  • Jérémie T. Zoueu
Laboratoire de Physique de la Matière Condensée et Technologie, Université Félix Houphouët-Boigny, Abidjan, Côte d’Ivoire

 https://orcid.org/0000-0001-7046-1150
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Department of Physics, University of Nairobi, PO Box 30197 – 00100, Nairobi, Kenya

 https://orcid.org/0000-0002-0394-6999
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Laboratoire d’Instrumentation, Image and Spectroscopie, Institut National Polytechnique Félix Houphouët-Boigny, Yamoussoukro, Côte d’Ivoire

 https://orcid.org/0000-0003-4588-1641
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 Corresponding Author
Laboratoire d’Instrumentation, Image and Spectroscopie, Institut National Polytechnique Félix Houphouët-Boigny, Yamoussoukro, Côte d’Ivoire and Laboratoire de Physique de la Matière Condensée et Technologie, Université Félix Houphouët-Boigny, Abidjan, Côte d’Ivoire
[email protected]
 https://orcid.org/0000-0001-9954-7484
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We report the use of Time Multiplexing Super Resolution (TMSR) to reduce significantly speckle noise in spectral imaging microscopy of unstained thin blood smear samples of malaria-infected blood. The method is based on combining speckle illumination with a moving array serving as an encoding mask. We propose the use of a new encoding mask to improve the performance of the conventional TMSR method. The new mask is a two-dimensional generalisation of the one-dimensional Ipatov code. The mask is projected on the object and 13 low-resolution images captured and subsequently decoded properly using the same array. The low contrast images are added and extracted from the resulting reconstruction, giving a super-resolved, high-contrast image. The Ipatov filter used in this work performs better than the Barker filter.

Keywords: image contrast, time multiplexing, transfer function, super resolution, image processing, blood smear, multispectral, speckle ­reduction, diffraction, malaria

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