Journal of Spectral Imaging,   Volume 8   Article ID a14   (2019)

Peer reviewed Paper

Preferred metabolic pathway of bovine muscle fibre revealed by synchrotron–deep ultraviolet fluorescence imaging

  • Thierry Astruc
  • Olivier Loison
  • Frédéric Jamme
  • Matthieu Réfrégiers
  • Annie Vénien
UR370 Qualité des Produits Animaux, INRA, 63122 Saint-Genès-Champanelle, France

 https://orcid.org/0000-0001-8167-7977
 Search for papers by this author
Synchrotron SOLEIL, BP48, L’Orme des Merisiers, 91120 Gif-sur-Yvette, France

 https://orcid.org/0000-0002-7398-7868
 Search for papers by this author
Synchrotron SOLEIL, BP48, L’Orme des Merisiers, 91120 Gif-sur-Yvette, France

 https://orcid.org/0000-0002-3281-2308
 Search for papers by this author
UR370 Qualité des Produits Animaux, INRA, 63122 Saint-Genès-Champanelle, France

 https://orcid.org/0000-0002-9270-2151
 Search for papers by this author
 Corresponding Author
UR370 Qualité des Produits Animaux, INRA, 63122 Saint-Genès-Champanelle, France
[email protected]
 https://orcid.org/0000-0001-9528-5827
 Search for papers by this author

The different bovine muscle fibre types I, IIA and IIX are characterised by their preferred metabolic pathway, either oxidative (I, IIA) or glycolytic (IIX), and their contraction speed, either slow-twitch (I) or fast-twitch (IIA, IIX). These physiological specificities are associated with variations in intracellular composition and their fluorescence spectra signatures. We hypothesised that these slight differences in autofluorescence responses could be used to discriminate the muscle fibre types by fluorescence imaging. Serial histological cross-sections of beef longissimus dorsi were performed: the start set was used to identify the metabolic and contractile type of muscle fibres by both immunohistoenzymology and immunohistofluorescence, and the following set was used to acquire synchrotron–deep ultraviolet (UV) autofluorescence images after excitation in the UV range (275 nm and 315 nm). This strategy made it possible to explore the label-free autofluorescence of muscle cells previously subtyped by histochemistry. Glycolytic cells (IIX) showed more intense fluorescence than oxidative cells (I and IIA) with near-90 % accuracy. This discrimination is more specifically assigned to the fluorescence of nicotinamide adenine dinucleotide. UV autofluorescence was unable to discriminate contractile type.

Keywords: skeletal muscle, fibre type, myosin isoform, UV microspectroscopy, histology, synchrotron radiation

Metrics

Downloads:

155

Full-Text HTML Views:

16

Abstract Views:

1,118